Nucleic Acids Hybridization Modern Applications [Paperback]

Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.

Contributing AuthorsPrefaceAcknowledgements Nucleic acids hybridization: potentials and limitationsAnton Buzdin Selective suppression of polymerase chain reaction and its most popular applicationsSergey Lukyanov, Konstantin Lukyanov, Nadezhda Gurskaya, Ekaterina Bogdanova, Anton Buzdin Suppression subtractive hybridizationSergey Lukyanov, Denis Rebrikov, Anton Buzdin Stem-loop oligonucleotides as hybridization probes and their practical use in molecular biology and biomedicineAnton Buzdin, Sergey Lukyanov Generation of normalized cDNA librariesAlex Shcheglov, Pavel Zhulidov, Ekaterina Bogdanova, Dmitry Shagin Primer extension enrichment reaction (PEER) and other methods for difference screeningLilia Ganova-Raeva Subtractive hybridization with covalently modified oligonucleotidesShi-Lung Lin, Donald Chang, Joseph Miller and Shao-Yao Ying Coincidence cloning: robust technique for isolation of common sequencesAnton Buzdin DNA hybridization in solution for mutation detectionAnton Buzdin Current attempts to improve the specificity of nucleic acids hybridizationAnton Buzdin Concepts on microarray design for genome and transcriptome analysesHelder I. Nakaya, Eduardo M. Reis and Sergio Verjovski-Almeida Index

In-depth review of nucleic acid hybridization in vitro techniques

All major applications covered

Includes novel methods with the potential of being widely used

Detailed experimental protocols provided

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