The volume provides detailed protocols that have been developed or modified exclusively for the study of oat. The topics discussed in this book are a selection of various molecular biology and biotechnology methods, such as the application of molecular markers for polymorphism analyses and cytological manipulations, the production of synthetic polyploids, and in vitro cultures and genetic modifications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Oat: Methods and Protocols is a useful resource in the development of new research approaches toward organizing the oat genome and the identification of new and useful traits for further improvements of this exceptional crop.
Preface&
Table of Contents&
Contributing Authors&
Part I Cytological Methods
1. Fluorescence In Situ Hybridization in Oat
Eva Wegel
2. Oat Doubled Haploids Following Maize Pollination
Philip A. Davies and Parminder K. Sidhu
3. Wide Hybridization between Oat and Pearl Millet
Takayoshi Ishii
4. Protocol for Producing Synthetic Polyploid Oats
Toshinobu Morikawa
Part II In Vitro Cultures and Genetic Transformation
5. Manipulation of Oat Protoplasts for Transient Expression Assays
Robyn Roberts, Jincan Zhang, Nicole Mihelish, Danielle Savino, Aur?lie M. Rakotondrafara
6. Oat Anther Culture and Use of DH-Lines for Genetic Mapping
Elina Kiviharju, Sirpa MlăD