This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience. This book shows readers how to identify the critical aspects of each methodological step, and how to determine the necessary level of complexity to address the particular research question. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Comprehensive and cutting-edge, Optogenetics: A Roadmap is a valuable guide for both the optogenetics novices and the experts.
Neuromethods Series Preface&
Preface&
Table of Contents&
Contributing Authors&
1. Users Guide to the Selection of Viral Vectors for Optogenetic Studies
K.R. Thompson and C. Towne
2. Cell Type-Specific Targeting Strategies for Optogenetics
Ofer Yizhar and Antoine Adamantidis
3. Molecular Engineering of Channelrhodopsis for Enhanced Control over the Nervous System
Andr? Berndt
4. Optogenetic Control of Intracellular Signaling: Class II Opsins
Erik Ellwardt and Raag D. Airan
5. Optogenetics in Stem Cell Research: Focus on the Central Nervous System
Johannes Boltze and Albrecht Stroh
6. Optogenetic Applications in the Nematode Caenorhabditis elegans
Katharina Elisabeth Fischer, Nathalie Alexandra Vladis, and kalÓË