The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field.? These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells.? Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors.
In its updated 2nd edition, this Methods in Molecular Biology book offers tools for identifying target genes, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, generation of pluripotent stem cells and more.
1. Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues
Marie P. Khoury, Virginie Marcel, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon
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2. Detecting p53 Isoforms at Protein Level
Virginie Marcel, Marie P. Khoury, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon
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3. Autophagy, Senescence and Apoptosis
Rachel W. Goehe, Molly L. Brislc¶
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