PCR Methods and Protocols [Hardcover]

This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field.  Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology,  and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.

Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, and Kabyl Zhambakin

2. Introduction on using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis

Ruslan Kalendar and Timofey V. Tselykh

3. Long Fragment Polymerase Chain Reaction

Eng Wee Chua, Simran Maggo, and Martin A. Kennedy

4. Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR

Maria Jorge Campos and Alberto Quesada

5.  Inverse PCR for Point Mutation Introduction

Diogo Silva, Gustavo Santos, M?rio Barroca, and Tony Collins

6. Synthesis of Fusion Genes for Cloning by Megaprimer-based PCR

Tatiana Q. Aguiar, Carla Oliveira, and Luc?lia Domingues

 

7. A Novel Platform for High-throughput Gene Synlƒ¼