Phenotypic Screening Methods and Protocols [Hardcover]

This volume provides a better understanding of the advancements in phenotypic readouts and improved disease models that generate novel biological insights and recapitulate clinically relevant biology. Chapters focus on phenotypic screening, the use of human cell models, microscopic approaches, assays to measure fat accumulation in C. elegans, the threat response in zebrafish, and protein-protein interactions in plant growth and development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Phenotypic Screening: Methods and Protocols aims to make phenotypic screening approaches more accessible to a wide array of researchers throughout the academic and biotech communities.

1.      Nutrient Stress Small-Molecule Screening Platform for Escherichia coli

Sara S. El Zahed, Garima Kumar, Madeline Tong, and Eric D. Brown

 

2.      Validation and Development of an Escherichia coli Riboflavin Pathway Phenotypic Screen Hit as a Small-molecule Ligand of the Flavin Mononucleotide Riboswitch

Carl J. Balibar, Artjohn Villafania, Christopher M. Barbieri, Nick Murgolo, Terry Roemer, Hao Wang, and John A. Howe

 

3.      Phenotypic Screening of Small Molecules with Anti-malarial Activity for Three Different Parasitic Life Stages

Nobutaka Kato, Sandra March, Sangeeta N Bhatia, and Matthias Marti