Phosphodiesterase Methods and Protocols [Paperback]

Study of Cyclic Adenosine Monophosphate Microdomains in CellsMarco Mongillo, Anna Terrin, Sandrine Evellin, Valentina Lissandron, and Manuela ZaccoloHigh-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D MicrodomainsJeffrey W. Karpen and Thomas C. RichCygnets: In Vivo Characterization of Novel cGMP Indicators and In Vivo Imaging of Intracellular cGMPAkira Honda, Carolyn L. Sawyer, Sharon M. Cawley, and Wolfgang R. G. DostmannHigh-Throughput Screening of Phosphodiesterase Activity in Living CellsThomas C. Rich and Jeffrey W. KarpenAssessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue ExtractsTh?r?se Keravis, Rima Thaseldar-Roumi?, and Claire LugnierLocalization of the Cyclic Guanosine 39,59-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ HybridizationWilma C. G. van Staveren and Marjanne Markerink-van IttersumDetermination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact CellsChen YanAdenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3BFaiyaz Ahmad, Linda H?rndahl, Yan Tang, Lena Stenson Holst, and Vincent C. ManganielloIdentification of Promoter Elements in the 5'-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B GeneHanguan Liu, Jing Rong Tang, Eva Degerman, and Vincent C. ManganielloPurification of PDE6 Isozymes From Mammalian RetinaDana C. Pentia, Suzanne Hosier, Rachel A. Collupy, Beverly A. Valeriani, and Rick H. CoteCyclic Guanosine 59-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor PhosphodiesteraseRick H. CoteRenaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion BodiesWito Richter, Thomas Hermsdorf, and Dietrich DettmerDetermining the SubunitStructure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient CentrifugationWito RichterCrystallization of Cyclic Nucleotide PhosphodiesterasesHengming Ke, Qing Huai, and Robert X. XuGeneration of PDE4 KnoclÃ