The Phospholipases [Paperback]

1 Assay of Phospholipases.- 1.1. General Considerations and Choice of Assay.- 1.1.1. Physical Form of Substrate.- 1.1.2. Liposomes.- 1.1.3. Emulsions.- 1.1.4. Monomolecular Films.- 1.1.5. Natural Cellular Membranes.- 1.1.6. Lipoproteins.- 1.2. Methods of Assay.- 1.2.1. Titration.- 1.2.2. Colorimetric and Fluorimetric Assays.- 1.2.3. Assay Employing Radiolabeled Substrates.- 1.2.4. Preparation of Fatty Acyl-Labeled Phospholipids.- 2 Bacterial Acyl Hydrolases (Phospholipases A, B, and Lysophospholipases).- 2.1. General Considerations.- 2.1.1. Escherichia coli.- 2.1.2. Bacillus megaterium.- 2.1.3. Mycobacterium phlei.- 2.1.4. Acyl Hydrolases of Genus Vibrio.- 3 Bacterial Phosphodiesterases (Phospholipases C and D).- 3.1. General Considerations.- 3.1.1. Genus Clostridium.- 3.1.2. Bacillus cereus.- 3.1.3. Staphylococcus aureus.- 3.1.4. Acinetobacter.- 3.1.5. Streptomyces hachijoensis.- 3.1.6. Genus Pseudomonas.- 3.2. Bacterial Phospholipases D.- 3.2.1. Genus Corynebacterium.- 3.2.2. Genus Streptomyces.- 3.2.3. Haemophilus parainfluenzae.- 3.2.4. Other Gram-negative Bacteria.- 3.2.5. Vibrio damsela.- 3.3. Summary.- 4 Other Microorganisms.- 4.1. General Comments.- 4.2. Penicillium notatum.- 4.3. Saccharomyces cerevisiae.- 4.3.1. Acyl Hydrolases (Phospholipase B).- 4.3.2. Phosphodiesterases (Phospholipases C and D).- 4.4. Amoebic Phospholipases.- 4.4.1. Naegleria fowleri.- 4.4.2. Acanthamoeba.- 4.5. Tetrahymena pyriformis.- 5 Plant Phospholipases.- 5.1. General Considerations.- 5.2. Initial Studies.- 5.3. Purification of Phospholipase D.- 5.4. Transphosphatidylation and Substrate Specificity.- 5.5. Factors Regulating Phospholipase D Action.- 5.6. Function of Plant Phospholipases D.- 6 Cellular Phospholipases Al and Lysophospholipases of Mammals.- 6.1. General Introduction to Cellular Phospholipases from Mammals.- 6.2. Phospholipase A1: General Considerations.- 6.2.1. Pancreatic Phospholipases Al.- 6.2.2. Liver.- 6.2.3. Heart.- 6.3. Lysophospholipases: General Considerationsl£¾