Photosynthesis Research Protocols [Hardcover]

The capture of sunlight by photosynthetic organisms supplies an enormous amount of the energy required to develop and sustain life on the planet, making photosynthesis one of the most important biological phenomenon on Earth. Updating the popular first edition, Photosynthesis Research Protocols, Second Edition presents detailed descriptions of a broad range of general and fundamental methods that are commonly used by plant biochemists, physiologists, and molecular biologists, all of which are contributed by leading researchers in the field. This vital new edition also features coverage of methods related to the most abundant protein on earth, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase. Written in the highly successful Methods in Molecular Biology series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Photosynthesis Research Protocols, Second Edition serves new photosynthesis researchers as well as experienced scientists seeking to use a new type of preparation or method to study this tremendously influential process.

Now in a new and fully updated edition, this book presents detailed descriptions of a plethora of general and fundamental photosynthesis methods used by plant biochemists, physiologists, and molecular biologists. All are contributed by leading researchers.

1. Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex (OEC) Subunit Proteins from Higher PlantsYasusi Yamamoto, Jing Leng, and Jian-Ren Shen2. Isolation of Photosystem I Submembrane FractionsJohanne Harnois, Najoua Msilini, and Robert Carpentier3. Isolation of Photosystem II Reaction Center Complexes from PlantsMichael Seibert and Rafael Picorel4. Methods for the Isolation of Functional Photosystem II Core Particles fl.