Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states.? In Planar Cell Polarity: Methods and Protocols, expert researchers in the field present a number of detailed and well-designed protocols used successfully in their labs around the world.? As a volume in the highly successful Methods in Molecular Biology series, the chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
?
Timely and authoritative, Planar Cell Polarity: Methods and Protocols serves as a valuable reference for both novices and experts in this dynamic and versatile area of study.
Planar Cell Polarity presents well-designed protocols used successfully around the world.? Chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and troubleshooting tips.
1. Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations
??????????? Daryl J.V. David, Melanie A. McGill, R.F. Andrew McKinley, and Tony J.C. Harris
?
2. Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell
??????????? Bertrand Jauffred and Yohanns Bellaiche
?
3. Protein-Protein Interaction Techniques: Dissect PCP Signaling in Xenopus
??????????? Yingqun Wang
?l#Ý