Plant Cell Culture Protocols [Hardcover]

This fourth edition provides new and updated protocols on plant cell, tissue, and organ cultures. Chapters are divided into five parts that cover topics from general methodologies, statistical analysis and contamination control, highly specialized techniques, and laborious process of measuring the epigenetics changes in tissue cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Plant Cell Culture Protocols, Fourth Edition aims to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

Part I: Introduction

1. An Introduction to Plant Tissue Culture: Advances and Perspectives

Victor M. Loyola-Vargas and Neftal? Ochoa-Alejo

 

Part II: Cell Culture the Fundaments 2. Micropropagation in the XXI Century Jean Carlos Cardoso, Lee Tseng Sheng Gerald, and Jaime A. Teixeira da Silva  

3. Cellular and Morpho-histological Foundations of in vitro Plant Regeneration Diego Ismael Rocha, Lorena Melo Vieira, Andr?a Dias Koehler, Wagner Campos Otoni   4. Bacterial Endophytes in Plant Tissue Culture: Mode of Action, Detection, and Control Mona Quambusch and Traud Winkelmann   5. Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture

Victor Gaba, Yehudit Tam, Danny Shavit, Benjamin S

 

6. Selection of Molecular Markers for the Estimation of Somaclonal Variation Octavio Mart?nez &l+