Plant Cell Culture Protocols [Paperback]

Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. Plant Cell Culture Protocols's state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.Part I. Introduction. An Introduction to Plant Cell Culture: Pointers to Success, R. D. Hall. Part II. Cell Culture and Plant Regeneration. Callus Initiation, Maintenance, and Shoot Induction in Rice, N. W. Blackhall, J. P. Jotham, K. Azakanandam, J. B. Power, K. C. Lowe, E. C. Cocking, and M. R. Davey. Callus Initiation, Maintenance, and Shoot Induction in Potato, R. F. Curry and A. C. Cassells. Somatic Embryogenesis in Barley Suspension Cultures, M. Kihara, H. Funatsuki, K. Ito, and P. A. Lazzeri. Somatic Embryogenesis in Picea Suspension Cultures, U. Egertsdotter. Specialized Techniques. Direct, Cyclic Somatic Embryogenesis in Cassava for Mass Production Purposes, K. J. J. M. Raemakers, E. Jacobsen, and R. G. F. Visser. Immature Inflorescence Culture of Cereals, S. Rasco-Gaunt and P. Barcelo. Cryopreservation of Rice Tissue Cultures, E. E. Benson and P. T. Lynch. Noncryogenic Long-Term Germplasm Storage, A. Golmirzaie and J. Toledo. Part III. Plant Propagation In Vitro. Microprogation of Strawberry via Axillary Shoot Proliferation, P. Boxus. Meristem-Tip Culture for Propagation and Virus Elimination, B. W. W. Grout. Specialized Techniques. Clonal Propagation of Orchids, B. Tisserat and D. Jones. In Vitro Propagation of SucculelC¨