Plant Cell Expansion Methods and Protocols [Paperback]

This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists.

1. Penium margaritaceum as a Model Organism For Cell Wall Analysis Of Expanding Plant Cells

Maja G. Rydahl, Jonatan U. Fangel, Maria Dalgaard Mikkelsen, I. Elisabeth Johansen, Amanda Andreas, Jesper Harholt, Peter Ulvskov, Bodil J?rgensen, David S. Domozych, ?and William G.T. Willats

?

2. Using Chemical Genomics to Study Cell Wall Formation and Cell Growth in Arabidopsis thaliana and Penium margaritaceum

N. Worden, V. Esteva Esteve, D. S. Domozych, and G. Drakakaki

?

3. Optimized Method For Growing In Vitro arabidopsis Thaliana Pollen Tubes

Cecilia Borassi, Juliana Perez Di Giorgio, Mar?a R. Scarpin, Jorge Muschietti, and? Jose Estevez

?

4. Imaging of Calcium Dynamics in Pollen Tube Cytoplasm

Mar?a Laura Barberl£Ù