?Plant Cell Morphogenesis: Methods and Protocols provides a collection of experimental techniques used in current research on the cellular aspects of plant morphogenesis.?Methods and techniques include contemporary takes on? classical light microscopy ?and ?histochemistry through automated microscopy applications, use of advanced optical tools, quantitative image analysis, study of cellular dynamics of apical meristems, specialized ?electron microscopy ?techniques, and methods ?used to study specific model plant cell types and protocols for using heterologous expression in yeast to study cell? morphogenesis genes. Individual chapters in the highly successful Methods in Molecular Biology series format are written by ?expert researchers in the field and ?include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
This book features cutting-edge methods and protocols in plant cell morphogenesis in the MiMB standard format. It provides step-by-step detail essential for reproducible results and contains expert implementation advice.
1. Essential? Methods of Plant Sample Preparation for Light Microscopy
Alea Soukup and Edita Tylov?
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2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy
Alea Soukup
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3. Resin Embedding, Sectioning and Immunocytochemical Analyses of Plant Cell Walls in Hard Tissues
Kieran J. D. Lee and J. Paul Knox
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4. Automated Microscopy in Forward Genetic Screening of Arabidopsis
Tereza Dobisov? and Jan Hej?tko
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5. Image Analysis: Basic Procedures for Description of Plant Structures on l³«
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