This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.
Preface&
Table of Contents&
Contributing Authors&
1.Multi-Parametric Screening in Arabidopsis thaliana Seedlings
Long Nguyen, Andrzej Drozdzecki, Vera Goossens, Bert De Rybel, Tom Beeckman, and Dominique Audenaert
2.Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress
Susan Fozard and Brian G. Forde
3.Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen
Elena Marusich, Roman Chuprov-Netochin, Yaroslav Neskorodov, Yana Mishutkina, Polina Volynchuk, Sergey Leonov, Yan Ivanenkov, Alisher Touraev, and Klaus Palme
4.A High-Throughput Chemical Screening Method for Inhibitors al3.
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