This volume provides a comprehensive collection of protocols that can be used to study plant chromatin structure and composition. Chapters divided into three sections detail the profiling of chromatin features in relation to epigenetic regulation, investigate the interaction between chromatin modifications and gene regulation, and explore the 3D spatial organization of the chromatin inside the nucleus. Written in the highly successful?Methods in Molecular Biology?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,?
Plant Chromatin Dynamics: Methods and Protocols?aims to ensure successful results in the further study of this vital field.
1. Section Overview: Profiling Developmentally and Environmentally Controlled Chromatin Reprogramming
Clara Bourbousse, Moussa Benhamed, and Fredy Barneche
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2. Profiling DNA Methylation using Bisulfite Sequencing (BS-Seq)
Yun-Ru Chen, Sheng Yu, and Silin Zhong
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3. Bisulfite Sequencing using Small DNA Amounts
Susanne Edelmann and Stefan Scholten
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4. Identification of Differentially Methylated Regions in the Genome of Arabidopsis thaliana
Kamal Kishore and Mattia Pelizzola
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5. A Rapid and Efficient ChIP Protocol to Profile Chromatin Binding Proteins and Epigenetic Modifications in Arabidopsis
B?n?dicte Desvoyes, Zaida Vergara, Joana Sequeira-Mendes, Sofia Madeira, and Crisanto Gutierrez
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6. Sequential ChIP Protocol for Profiling Bivalent Epigenetic Modilı
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