Plant Cold Acclimation Methods and Protocols [Hardcover]

Plant Cold Acclimation: Methods and Protocols details many of the methods and protocols commonly used to study plant cold acclimation and freezing tolerance, breeding, genetics, physiology or molecular biology, or any combination of these specialties. Chapters focus on interdisciplinary approaches, experimental methods, and concepts from different areas of science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Cold Acclimation: Methods and Protocols seeks to help not only new researchers starting in this field, but also those already working in a particular area of cold acclimation and freezing tolerance research who are looking to expand their range of experimental approaches.

1. Measuring Freezing Tolerance: Survival and Regrowth Assays

Daniel Z. Skinner and Kimberly Garland-Campbell

?

2. Measuring Freezing Tolerance: Electrolyte Leakage and Chlorophyll Fluorescence Assays

Anja Thalhammer, Dirk K. Hincha and Ellen Zuther

?

3. Conducting Field Trials for Freezing Tolerance Breeding in Cereals

?Luigi Cattivelli

?

4. A Whole Plant Screening Test to Identify Genotypes with Superior Freezing Tolerance

Annick Bertrand, Yves Castonguay and Jos?e Bourassa

?

5. Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation

Evelyne T?oul? and Carine G?ry

?

6. Common GardlÓ4