This volume covers arange of methods used in plant cytogenetics, beginning with basic analysis ofchromosomes and visualizing gene locations, to manipulating and dissectingchromosomes, and then focusing on less understood features of chromosomes suchas recombination initiation sites and epigenomic marks. The methods describedin Plant Cytogenetics: Methods and Protocols build on each other andprovide, those new to the field, with a comprehensive platform to support theirresearch endeavours, while also introducing advanced techniques to experiencedresearchers. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Cuttingedge and thorough, Plant Cytogenetics: Methods and Protocols, is avaluable resource for anyone who is interested in the diverse and wonderfullycomplex field of cytogenetics.
1. C-Banding of Plant Chromosomes
Eric N. Jellen
2. Chromosome Painting by GISH and Multi-ColorFISH
Steven S. Xu, Zhao Liu, Qijun Zhang,Zhixia Niu, Chao-Chien Jan, and Xiwen Cai
3. Fluorescent In Situ Hybridization on Extended Chromatin Fibers forHigh-Resolution Analysis of Plant Chromosomes
Daryna Dechyeva and Thomas Schmidt
4. Tyramide Signal Amplification: FluorescenceIn Situ Hybridization for IdentifyingHomologous Chl“%