The Plant Endoplasmic Reticulum Methods and Protocols [Hardcover]

This volume presents a range of different techniques that have been used to?characterize?the structure and function of the endoplasmic reticulum (ER) in higher plants. Chapters guide readers through application of modern microscopy techniques by fluorescence and electron microscopy, new protocols for analysing ER network structure, methods to purify and analyse ER membrane structure and to study protein glycosylation, protocols to study the unfolded protein response, and the role of the ER in autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

?Authoritative and cutting-edge, The Plant Endoplasmic Reticulum: Methods and Protocols aims to ensure successful results in the further study of this vital field.?

1. Labelling the ER for Light and Fluorescence Microscopy

Chris Hawes, Pengwei Wang, and Verena Kriechbaumer

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2. 3D Electron Microscopy of the ER

Maike Kittelmann

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3. Characterization of Proteins Localised to Plant ER-PM Contact Sites

Pengwei Wang, Chris Hawes, and Patrick J. Hussey

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4. Preparation and Imaging of Specialised ER using Super-resolution and TEM Techniques

Karen Bell, Karl Oparka and Kirsten Knox

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5. Quantitation of ER Structure and Function

Mark Fricker, Luke Heaton, Nick Jones, Boguslaw Obara, Stefanie J. M?ller, and Andreas J. Meyer

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6. Long-term Imaging of Endoplasmic Rl“.