Plant Epigenetics and Epigenomics Methods and Protocols [Hardcover]

Many fundamental discoveries concerning epigenetics and the elucidation of mechanisms of epigenetic regulation have developed from studies performed in plants. In Plant Epigenetics and Epigenomics: Methods and Protocols, leading scientists in the epigenetics field describe comprehensive techniques that have been developed to understand the plant epigenetic landscape.?These include recently developed methods and techniques for analysis of epigenetically regulated traits, such as flowering time, transposon activation, genomic imprinting and genome dosage effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoidance of known pitfalls.

Authoritative and practical, Plant Epigenetics and Epigenomics: Methods and Protocols seek to aid scientists in the further study of plant epigenetic phenomena using advanced contemporary methods.

1. Landscaping Plant Epigenetics

Peter C. McKeown and Charles Spillane

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2. The Gene Balance Hypothesis: Dosage Effects in Plants

James A. Birchler and Reiner A. Veitia

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3. High-throughput RNA-Seq for Allelic or Locus-specific Expression Analysis in Arabidopsis Related Species, Hybrids, and Allotetraploids

Danny W-K. Ng, Xiaoli Shi, Gyoungju Nah, and Z. Jeffrey Chen

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4. Inference of Allele-specific Expression from RNA-seq Data

Paul K. Korir and Cathal Seoighe

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5. Screening for Imprinted Genes using High Resolution Melting Analysis of PCR Amplicons

Robert Day anl%