Plant Epigenetics Methods and Protocols [Paperback]

Thisvolume provides a variety of protocols to analyze various epigeneticchanges, including differential expression of non-coding RNAs, changes in DNAmethylation, and histone modifications in plants. Chapters detail protocolswith different degrees of complexity, and describe bioinformatics approachesfor data processing and analysis. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

Authoritative andcutting-edge, Plant Epigenetics: Methods and Protocols, Second Editionaims to ensure successful results in the further study of this vital field.

1. Chromatin ImmunoprecipitationProtocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis

Wanhui You, St?phane Pien, and UeliGrossniklaus

 

2.Chromatin Conformation Capture-Based Analysis of Nuclear Architecture

 

3.Metaanalysis of Genome-Wide Chromatin Data

 

4.Localization of Mirnas by In Situ Hybridization in Plants using ConventionalOligonucleotide Probes

 

5.COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis ofLocus-Specific Changes in Methylation Patterns

 

6.Analysis of Global Genome Methylation Using the Cytosine-Extension Assay

AndriyBilichak and Igor Kovló-