Plant Genomics Methods and Protocols [Paperback]

This volume provides protocols that revolve around three pillars of progress in the plant genomics field: genotypes, phenotypes, and the molecular processes in between. Chapters in Plant Genomics: Methods and Protocols are not restricted to the predominant model species Arabidopsis thaliana, hoping to encourage and facilitate other researchers to expand their research to other species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Part I: Genotypes

1. CRISPR/Cas-mediated in planta Gene Targeting

Simon Schiml, Friedrich Fauser, and Holger Puchta

 

2. User Guide for the LORE1 Insertion Mutant Resource

Terry Mun, Anna Malolepszy, Niels Sandal, Jens Stougaard, and Stig U. Andersen

 

3. Enabling Reverse Genetics in Medicago truncatula Using High Throughput Sequencing for Tnt1 Flanking Sequence Recovery

Xiaofei Cheng, Nick Krom, Shulan Zhang, Kirankumar S. Mysore, Michael Udvardi, and Jiangqi Wen

  4. Generation of Doubled Haploid Lines for QTL Mapping

Daniele L. Filiault, Danelle K. Seymour, Ravi Maruthachalam, and Julin N. Maloof

 

 

Part IIlsh