Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation.? In plants, meiotic recombination is by far the most important source of genetic variation.? In?Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants.? These state-of -the-art protocols allow studying the organization and behavior of the genetic material in a wide range of both model and crop species. ?Written in the highly successful?Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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?Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.
This Methods in Molecular Biology book offers methods for molecular cytogenetics and chromosome analysis in plants. Includes state-of-the-art protocols for studying the organization and behavior of genetic material in a wide range of model and crop species.
Section I: CYTOLOGICAL TECHNIQUES FOR LIGHT MICROSCOPY
1. Spreading and Fluorescence in situ Hybridization (FISH) of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenenetical Analysis
Susan Armstrong
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2. Analysis of Plant Meiotic Chromosomes by Chromosome Painting
Martin A. Lysak and Terezie Mand?kov?
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3. Using Sequential Fluorescence and Genomic in situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in lăd
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