Plant Metabolism Methods and Protocols [Hardcover]

In Plant Metabolism: Methods and Protocols, expert researchers in the field present the latest methods on quantitative analysis of plant metabolism. The methods focus on measurements, analyses and simulations of molecules, fluxes, and ultimately entire metabolic pathways and networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, reagents, or software, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Metabolism: Methods and Protocols seeks to benefit scientists ranging from plant biology, metabolic engineering, and biotechnology.

Part I: Metabolites and Metabolic Fluxes

1. MeRy-B, a Metabolomic Database and Knowledge Base for Exploring Plant Primary Metabolism

Catherine Deborde and Daniel Jacob

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2. Targeted Deuteration of Polyphenolics for their Qualitative and Quantitative Metabolomic Analysis in Plant-Derived Extracts

Mikel R. Roe, Jerry D. Cohen, and Adrian D. Hegeman

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3. Relative Quantitation in Single Cell Metabolomics by Laser Ablation Electrospray Mass Spectrometry

Bindesh Shrestha and Akos Vertes

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4. Quantification of Plant Volatiles

Anthony V. Qualley and Natalia Dudareva

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5. Quantitative Imaging Approaches for Small Molecule Measurements using FRET Sensors in Plants

Sakiko Okumoto

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6. Isotopomer Measurement Techniques in Metabolic Flux Analysis I: Nuclear Magnetic Resonance

Quyen X. Truong, Jong Moon Yoon, and? Jacqueline V. Sl“}