Plant Nitric Oxide Methods and Protocols [Paperback]

With numerous methods available for the quantification ofnitric oxide (NO), this detailed book explores their advantages anddisadvantages in order to provide a foundation for further research in plantnitric oxide. After providing a useful practical guide to choosing a techniquefor measuring NO from plant materials, the book continues with chapters on chemiluminescence,diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well asmany other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,step-by-step, readily reproducible laboratory methods, and tips on troubleshootingand avoiding known pitfalls.

Practical and authoritative, PlantNitric Oxide: Methods and Protocols serves as a valuable guide to allresearchers working and intending to work in the field of plant nitric oxideresearch.

1. An Overview ofMethods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use MultipleMethods?

            HideoYamasaki, Naoko S. Watanabe, Yasuko Sakihama, and Michael F. Cohen

 

2. Chemiluminescence Detection ofNitric Oxide from Roots, Leaves, and Root Mitochondria

            Aakanksha Wany, Alok K .Gupta, , Aprajita Kumari,  Shika Gupta,Sonal Mishra, Ritika Jaintu, Pradeep K. Phatak, and Kapuganti Jagadis Gupta

 

3. NitricOxide Measurement from Purified Enzymes and Estimation of Scavenging Activityby Gas Phase Chemlimunimescence Method