Plant Protoplasts and Genetic Engineering II [Paperback]

I Genetic Engineering and In Vitro Manipulation of Plant Cells  Technical Advances.- 1 Introduction.- 2 Somatic Hybrids and Cybrids.- 3 Recombinant DNA Technology and Transformation.- 4 Uptake and Incorporation of Organelles, Viruses, Bacteria, and Fungi by Protoplasts.- 5 Newer Techniques for Genetic Engineering.- 6 Conclusions.- References.- II Genetic Manipulation: Generative Versus Somatic.- 1 Introduction.- 2 The Structure of a Plant Breeding Program.- 3 Construction and Isolation of a Desired Genotype.- 4 Reproductive Systems.- 5 Conclusions and Prospects.- References.- III Uptake and Integration of Exogenous DNA in Plants.- 1 Introduction.- 2 A Brief History of Plant Genetic Transformation.- 3 Uptake of Purified DNA by Seeds, Seedlings, and Plant Cells.- 4 Uptake of Purified DNA by Gametes.- 5 Uptake of Purified DNA by Protoplasts.- 6 Nature of Expression Vectors Used in Direct Gene Transfer.- 7 Integration of Foreign DNA Taken up by Protoplasts.- 8 Present Status and Conclusions.- References.- IV DNA Recombinants and Transformation of Agricultural Crops.- 1 Introduction.- 2 The Agrobacterium System.- 3 Vector Systems.- 4 Transfer and Expression of Foreign DNA in Plant Cells.- 5 Use of Selectable Reporter Genes in Plant Cells.- 6 Plant Transformation Systems.- 7 Use of Reverse Genetics Techniques to Study Plant Gene Regulation.- 8 Variability of Transformed Plants.- 9 Conclusions.- References.- V Genetically Transformed Maize (Zea mays L.) Plants from Protoplasts.- 1 Introduction.- 2 Methodology and Experimental Results.- 3 Summary and Conclusions.- References.- VI Protoplast Culture and Transformation Studies on Rice.- 1 Introduction.- 2 Isolation and Culture of Rice Protoplasts.- 3 Recent Progress in Culture and Regeneration of Rice Protoplasts (19841987).- 4 Transformation of Rice Protoplasts by Spheroplast Treatment.- 5 Introduction and Expression of Foreign Genes in Gramineae Plants.- 6 Summary and Conclusion.- References.- VII Transformation in PotatolD