Plant Receptor Kinases Methods and Protocols [Hardcover]

This is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways. Written in the highly successful Methods

in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Receptor Kinases: Methods and Protocols aims to ensure successful results in the further study of this vital field.

PART I Expression and Purification of PRKs

1. Expression of Plant Receptor Kinases in E. coli 

 

2. Expression of Plant Receptor Kinases in Yeast

Mar?a Laura Barberini and Jorge Muschietti

 

3. Expression of Plant Receptor Kinases in Tobacco BY-2 Cells

Hidefumi Shinohara and Yoshikatsu Matsubayashi

 

4. Cell-free Synthesis of Plant Receptor Kinases

 

5. Purification of Plant Receptor Kinases from Plant Plasma Membranes

Jin Suk Lee