Plant Respiration and Internal Oxygen Methods and Protocols [Hardcover]

This volume covers a wide range of methods to measure cellular respiration and internal oxygen in various tissues under different conditions. Chapters guide readers through informative experimental approaches, calorespirometry,?isotope fractionation techniques, protocols for?dual-inlet isotope ratio mass spectrometry,?laser-capture microdissection, and?bioinformatics approach for exploring the co-regulation of AOX gene family members.?Written in the highly successful?Methods in Molecular Biology?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical,?Plant Respiration and Internal Oxygen: Methods and Protocols?aims to be helpful for all students and researchers interested in the determination of respiration and internal oxygen.?

1. Assessing Metabolic Flux in Plants with Radiorespirometry

Nicholas J. Kruger, Shyam K. Masakapalli, and R. George Ratcliffe

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Toshihiro Obata, Laise Rosado-Souza, and Alisdair R. Fernie

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3. A Method for Imaging Oxygen Distribution and Respiration at a Microscopic Level of Resolution

?Hardy Rolletschek and Gregor Liebsch

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4. VisiSens Technique to Measure Internal Oxygen and Respiration in Barley Roots

Aprajita Kumari and Kapuganti Jagadis Gupta

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5. MultiSense: A Multimodal Sensor Tool Enabling the High Throughput Analysis of Respiration

Peter Keil, Gregor Liebsch, Ljudmilla Borisjuk, and Hardy Rolletschek