Plant Synthetic Promoters Methods and Protocols [Hardcover]

This book assembles experimental and bioinformatics protocols for the design and experimental testing of synthetic promoters. The identification of cis-regulatory elements potentially achieving the desired expression of a gene is at the core of synthetic promoter design. For this, several bioinformatics chapters are presented. The experimental verification of the proposed expression profile conferred by the cis-regulatory elements requires the assembly of synthetic promoters. Several chapters are dedicated to the assembly of synthetic promoters, also including specific software tools to facilitate promoter design. Transient and transgenic reporter gene technology is a prominent approach to test the spatial and temporal expression driven by synthetic promoters, and several chapters address this approach. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and expert implementation advice to ensure successful results in the lab. 

1. What Have We Learned About Synthetic Promoter Construction?

Paul J. Rushton

2. Quantitative Analysis of Cis-Regulatory Element Activity Using Synthetic Promoters in Transgenic Plants

Geoffrey Benn and Katayoon Dehesh

3. The Identification of Cis-Regulatory Sequence Motifs in Gene Promoters Based on SNP Information

Paula Korku and Dirk Walther

4. Quantitative Analysis of Protein-DNA Interaclsl