This detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors. Beginning with a brief introduction, the volume continues by exploring methods in the preparation of plant materials, detection of expression levels, interaction tests, and chromatin analyses. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Transcription Factors: Methods and Protocols aims to answer a wide range of questions related to transcription factors commonly raised by plant biologists.
Part I: Review and Overview Chapters
1. Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective
Kerstin Kaufmann and Chiara A. Airoldi
2. Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants
Felix Wolter and Holger Puchta
3. The Long-Term In Natura Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments
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