Plant Vacuolar Trafficking Methods and Protocols [Hardcover]

This volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a  highly valuable resource for researchers interested in learning more about this field

1. Green-fluorescent Seed, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds

Tomoo Shimada,  Kentaro Fuji, Takuji Ichino; Ooi-Kock The, Yasuko KoumotoIkuko, and Hara-Nishimura

 

2. Detection of CRISPR/Cas9 Induced Genomic Fragment Deletions in Barley and Generation of Homozygous Edited Lines via Embryogenic Pollen Culture

Eszter Kapusi and Eva St?ger

 

Bruno Peixoto, Susana Pereira, Cl?udia Pereira, and Jos? Pissarra

 

4. Vacuole-targeted Proteins: Ins and Outs of Subcellular Localization Studies

In?s Carqueijeiro, Liuda J. S?pulveda, Angela Mosquera, Richard Payne, Cyrielle Corbin, Nicolas Papon, Thomas Dug? de Bernonville, S?bastien Besseau,  Arnaud Lanoue, Ga?lle Gl?varec, Marc Clastre, Benoit St-Pierre; Lucia Atehort?a, Nathalie Giglioli-Guivarch, Sarah E. OConnor, Audrey Oudin, and Vincent Courdavault

 

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