Plant Virology Protocols New Approaches to Detect Viruses and Host Responses [Paperback]

Plant Virology Protocols: New Approaches to Detect Viruses and Host Responses addresses recent developments in genome analyses and cytological technologies being used today to learn more about plant virology. Opening with chapters covering techniques relevant to the detection of unknown viruses and disease diagnosis, this detailed volume continues with chapters on the utilization of meta-genome sequencing and global gene expression analyses for the search and identification of viruses, as well as the elucidation of host responses to viral infection, construction methods of infectious cDNAs, and methods relevant to plant virus control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

1. Detection of Plant Viruses in Mixed Infection by a Macroarray-Assisted Method

??????????? Hanako Shimura, Kazuyoshi Furuta, and Chikara Masuta

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2. RT-PCR and Real-Time RT-PCR Methods for the Detection of Potato Virus Y in Potato Leaves and Tubers

??????????? Tyler D. B. MacKenzie, Xianzhou Nie, and Mathuresh Singh

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3. New Method to Isolate Total dsRNA

??????????? Go Atsumi, Ken-Taro Sekine, and Kappei Kobayashi

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4. Multiplex RT-PCR Method for the Simultaneous Detection of Nine Grapevine Viruses

??????????? Giorgio Gambino

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5. Detection MetlcÈ