Poliovirus Methods and Protocols [Paperback]

This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Poliovirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. An Introduction to Poliovirus: Pathogenesis, Vaccination and the Endgame for Global Eradication

Philip D Minor

2. Poliovirus Laboratory Based Surveillance: An Overview

Syed Sohail Zahoor Zaidi, Humayun Asghar, Salmaan Sharif, and Muhammad Masroor Alam

3. Isolation and Characterisation of Enteroviruses from Clinical Samples

Soile Blomqvist and Merja Roivainen

4. Isolation and Characterisation of Poliovirus in Cell Culture Systems

Bruce R. Thorley and Jason A. Roberts

5. Molecular Characterization of Polio from Environmental Samples  ISSP, The Israeli Sewage Surveillance Protocol

Lester M. Shulman, Yossi Manor, Musa Hindiyeh, Danit Sofer, and Ella Mendelson

6. Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays

Glynis Dunn

7. A Transgenic Mouse Model of Poliomyelitis

Satoshi Koike and Noriyo Nagata

8. Standardized Methods for Detection of Poliovirus Antibodies

William C. Weldon, M. Steven Oberste, and Mark A. Pallanscl3%