Polyadenylation Methods and Protocols [Paperback]

In Polyadenylation: Methods and Protocols, expert researchers in the field detail many of the protocols which are now commonly used to study polyadenylation. Focusing on recent advances in the fast-moving polyadenylation filed, that has recently been recognized as a key contributor to the complexity of mammalian gene expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

PART I: SECTION I: 3-END PROCESSING IN EUKARYOTES

1. 3 end Formation and Regulation of Eukaryotic mRNAs

Thomas J. Sweet and ?Donny D. Licatalosi

PART II: I-A General Methods

2. ?Poly(A) Polymerase-based Poly(A) Length Assay

Deepak P. Patil, Baskar Bakthavachalu, ?and Daniel R. Schoenberg

3. Using Klenow Mediated Extension to Measure Poly(A)-tail Length and Position in the Transcriptome

Man C. Lee, Amrei J?nicke, and Traude H. Beilharz

4. Detection of Uridylated mRNAs

Fran?ois M. Sement, Dominique Gagliardi

5. Cytoplasmic Polyadenylation Assays

Olga Coll, Ana Villalba, F?tima Gebauer

6. In vitro Analysis of Transcriptional Activators and Polyadenylation

Takashi Nagaike and James L. Manley

7. Fluorescence Detection of Polyadenylation Reaction through the Coordination of Adensoine₂-coralyne-adensoine₂