Polyadenylation in Plants Methods and Protocols [Paperback]

This volume presents the most recent studies on mRNA polyadenylation in plants. Chapters are divided into three sections covering recent development of the use of bioinformatics tools in the field. numerous molecular, biochemical, and methods used to characterize polyadenylation sites on a genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.?Some are specific for plant research, but most can be adopted for research in other organisms.

Authoritative and practical, Polyadenylation in Plants: Methods and Protocols provides scientists with a wide range of methods to study mRNA 3-end formation in plants.

Part I: Bioinformatics Studies of Plant Polyadenylation

1. Computational Analysis Of Plant Polyadenylation Signals

Xiaohui Wu, Guoli Ji, ?and Qingshun Quinn Li

2. Prediction of Plant mRNA Polyadenylation Sites

Xiaohui Wu, Guoli Ji, ?and Qingshun Quinn Li

4. Poly(A)-tag Deep Sequencing Data Processing To Extract poly(A) Sites

Xiaohui Wu, Guoli Ji, ?and Qingshun Quinn Li

5. Analysis of poly(A) Site Choice Using A Java-Based Clustering Algorithm

Patrick E. Thomas

6. RADPRE: a Computational Program for Identification of Differential mRNA Processing Including Alternative Polyadenylation

Denghui Xing ?and Qingshun Quinn Li?

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