This volume provides readers with a collection of the latest protocols used by researchers to study polyamines (PA). The chapters in this book cover various topics, such as quantification of different polyamines and conjugates, subcellular localization studies, transport, DNA methylation, ODC regulation, genetic and phenotyping analyses, genome-wide association mapping, polyamine applications and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Polyamines: Methods and Protocols is a useful reference for researchers looking to advance and stimulate their knowledge of polyamines.
Preface&
Table of Contents&
Contributing Authors&
1. Polyamines in the Context of Metabolic Networks
Wegi Wuddineh, Rakesh Minocha, and Subhash C. Minocha
2. Molecules for Sensing Polyamines and Transducing their Action in Plants
Tomonobu Kusano, GHM Sagor, and Thomas Berberich
3. Polyamine Metabolism Responses to Biotic and Abiotic Stress
Fernando M. Romero, Santiago J. Maiale, Franco R. Rossi, Maria Marina, Oscar A. Ruiz, and Andr?s G?rriz
4. Thermospermine: An Evolutionary Ancient but Functionally New Compound in Plants
Taku Takahashi
5. Determination of Polyamines by Capillary Electrophoresis using Salicylaldehyde-5-Sulfonate as a Derivatizing Reagent
Takashi Kaneta
6. Detection of Thermospermine and Spermine by HPLC in Plants
Taku Takahashi, Ayaka Takano, and Jun-Ichi Kakehi
![Polyamines Methods and Protocols [Hardcover]](/itemImage/2/9/9/7/2_894604.jpg)