Post-translational Modifications of Proteins Tools for Functional Proteomics [Paperback]

This is a fully up-dated and expanded practical guide to protein structure-function relationships. This important area of research is brought up-to-date by the leading scientists in the field. The compilation of detailed protocols focuses on protein function, proteome research and characterization of pharmaceutical proteins, while following the successful format of the Methods in Molecular Biology series. Comprehensive and cutting edge, the book serves as practical guide for researchers working in the field of protein structure-function relationships and the rapidly growing field of proteomics, as well as scientists in the pharmaceutical industries.

This is a fully up-dated and expanded practical guide to protein structure-function relationships. The book is the most comprehensive volume on PTM analysis available. This important area of research is brought up-to-date by the leading scientists in the field.

Contents Preface Contributors 1 Disulfide Bond Mapping by Cyanylation-Induced Cleavage and Mass Spectrometry Jiang Wu 2 Detection of Post-Translational Modifications by Fluorescent Staining of 2D gels Archana M. Jacob and Chris W. Turck 3 Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods Christoph Weise and Christof Lenz 4 Analysis of Tyrosine-O-Sulfation Jens R. Bundgaard, Jette W. Sen, Anders H. Johnsen, and Jens F. Rehfeld 5 a -Amidated Peptides: Approaches for Analysis Gregory P. Mueller and William J. Driscoll 6 Gamma-Glutamate and b -Hydroxyaspartate in Proteins Francis J. Castellino, Victoria A. Ploplis, and Li Zhang 7 Lysine Hydroxylation and Cross-linking of Collagen Mitsuo Yamauchi and Masashi Shiiba 8 Mass Spectrometric Determination of Protein Ubiquitination Carol E. Parker, Maria R. E. Warren, Viorel Mocanu, Susanna F. Greer, and Christoph H. Borchers 9 Analysis of Sumoylation Andrea Pichler 10 Detection and Analysis of Protein ISGylation Tomoharu Takeuchi and Hideyoshi Yokosawa 11 AlF