Prions Methods and Protocols [Hardcover]

This volume details protocols on prion disease from multiple disciplines and highlights the contribution each discipline has made to the understanding of prion disease. Written in the highly successful?Methods in Molecular Biology?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical,?Prions: Methods and Protocols?aims to ensure successful results in the further study of this vital field.

PART I: Methods for Biochemical and Biophysical Analysis of Prion Disease

1. Purification and Fibrillation of Full-length Recombinant PrP Natallia Makarava, Regina Savtchenko, and Ilia V. Baskakov

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2. Method for Folding of Recombinant Prion Protein to Soluble b-sheet Secondary Structure

Laura J. Ellett

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3. Analysis of Prion Protein Conformation using Circular Dichroism Spectroscopy

Laura J Ellett and Vanessa Johanssen

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4. Analysis of Prion Protein Structure using Nuclear Magnetic Resonance Spectroscopy

Ivana Biljan, Gregor Ilc, and Janez Plavec

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5. Immunodetection of PrP^Sc using Western Immunoblotting Techniques

Gerald S. Baron and Gregory J. Raymond

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6. Analysis of miRNA Signatures in Neurodegenerative Prion Disease

Shayne A. Bellingham and Andrew F. Hill

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PART II: Methods for Cellular Analysis Of Prion Disease

7. Cell Biology Approaches to Studyinl#Ý