Progenitor Cells Methods and Protocols [Paperback]

Progenitor cells have become important in regenerative medicine therapies, due to their potential to differentiate into many cell types.? This capability, and understanding how to regulate the cells, will provide the basis for future cell therapies aimed at correcting tissue and organ dysfunction as a result of disease or injury. In, Progenitor Cells: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to investigate progenitor cells. These include methods and techniques of the manipulation of physical forces that shape progenitor cell behavior, studying progenitor cells in vivo, using non-mammalian and mammalian model systems, and investigating human progenitor cells, including their isolation, characterization and application in cell-based therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Progenitor Cells : Methods and Protocols seeks to aid scientists in the further study progenitor cells and how they are studied across multiple systems.

Part I.? In vitro Culture Systems

1. Isolation of Satellite Cells from Single Muscle Fibres from Young, Aged or Dystrophic Muscles

Valentina Di Foggia and Lesley Robson

2. Terminal Differentiation of Human Epidermal Stel#O