The Prostaglandins Volume 3 [Paperback]

1 Gas Chromatography-Mass Spectrometry of Prostaglandins.- I. Introduction.- A. Identification of Prostaglandin Synthetase Dependent Substances.- B. Metabolites of Prostaglandins.- C. Quantification.- II. Mass Spectrometry Systems for Prostaglandin Analysis.- A. Magnetic Focusing Instruments.- B. Quadrupole Instruments.- C. Choice of Internal Standard and Derivative.- III. Methods of Extraction and Chromatography.- A. Extraction of PGs, Their Circulating Metabolites, and Thromboxane B2 from Blood Plasma.- B. Extraction of Prostaglandins from Urine.- C. Methods of Chromatography of Prostaglandins and Prostaglandin Metabolites.- IV. Biological Applications.- A. Circulating Prostaglandins.- B. Urinary Prostaglandins.- C. Prostaglandins and Renin.- D. Assessment of Prostaglandin Synthesis.- V. References.- Note Added in Proof.- 2 Prostaglandin Biosynthesis and Metabolism as Measured by Radioimmunoassay.- I. Introduction.- II. Serological Specificities of the Prostaglandin Immune Systems.- A. Prostaglandin Synthetase.- B. Prostaglandin E 9-Ketoreductase.- C. 15-Hydroxyprostaglandin Dehydrogenase.- D. Prostaglandin ?13-Reductase.- E. PGE Dehydrase and PGA and PGC Isomerases.- III. Prostaglandin Synthetase.- A. Cofactor Requirements.- B. Activation and Inhibition of Microsomal Prostaglandin Biosynthesis by Cytoplasmic Fractions.- C. Solubilization and Resolution of a Prostaglandin Synthetase System.- D. The pH Optima of Prostaglandin Synthetase Systems.- E. Inhibition of Prostaglandin Synthetase from Different Tissues of the Same Species by Nonsteroidal Anti-inflammatory Drugs.- IV. Prostaglandin E 9-Ketoreductases.- A. Cofactor Requirements and Tissue Distribution of PGE 9-Ketoreductase.- B. Purification and Properties.- V. 15-Hydroxyprostaglandin Dehydrogenases.- A. Detection and Tissue Distribution of NAD+-Dependent and NADP+ Dependent 15-Hydroxyprostaglandin Dehydrogenases.- B. Purification and Properties of the NAD+- and NADP+- Dependent Dehydrogenases.- VI. ProstaglalÓÕ