Protein Crystallography Methods and Protocols [Paperback]

This volume provides methods for modern macromolecular crystallography, including all steps leading to crystal structure determination and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Protein Crystallography aims to ensure successful results in the further study of this vital field.

1. Expression and Purification of Recombinant Proteins in Escherichia Coli with a His₆ or Dual His₆-MBP Tag

Sreejith Raran-Kurussi and David S. Waugh

 

2. Protein Crystallization

Alexander McPherson

 

3. Advanced Methods of Protein Crystallization

Abel Moreno

 

4. The Sticky Patch Model of Crystallization and Modification of Proteins for Enhanced Crystallizability

Zygmunt S. Derewenda and Adam Godzik

 

5. Crystallization of Membrane Proteins: and Overview

Andrii Ishchenko, Enrique Abola, and Vadim Cherezov

 

6. Locating and Visualizing Crystals for X-ray Diffraction Experiments

Michael Becker, David J. Kissick, and Craig M. Ogata

 

7. Collection of X-ray Diffraction Data from Macromolecular Crystals

Zbigniew Dauter

 

8. Identifying and Overcoming Crystal Pathologies: Disorder and Twinning

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