This volume presents detailed protocols for novel strategies and approaches to improve functional understanding of protein N- and C-terminal biology. Protein Terminal Profiling: Methods and Protocols addresses topics such as protease specificity profiling, N-terminal acetylation, assays to probe protease activity in cellular systems, protein N- and C-termini on a proteome-wide scale, and biochemical approaches to explain and examine extracellular protease activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Cutting-edge and thorough, Protein Terminal Profiling: Methods and Protocols is a valuable resource for researchers that focus on biochemistry and cell biology, and those who share a broad interest in protein functionality and protein modifications.
Preface&
Table of Contents&
Contributing Authors&
1. [14C]-Acetyl-CoenzymeA-Based In Vitro N-Terminal Acetylation Assay
Adrian Drazic and Thomas Arnesen
2. DTNB-Based Quantification of In Vitro Enzymatic N-Terminal Acetyltransferase Activity
H?vard Foyn, Paul R. Thompson, and Thomas Arnesen
3. SILProNAQ: A Convenient Approach for Proteome-Wide Analysis of Protein N-Termini and N-Terminal Acetylation Quantitation
Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel
4. Profiling of Protein N-lóä