Proteome and Protein Analysis [Paperback]

The papers selected here cover new, sensitive and rapid methods for the analysis of proteins, with special emphasis on the proteome. In addition to the experimental details, the advantages and limitations of the methodological approaches are discussed, and topics include sequencing analysis, sample preparation, mass spectrometry, NMR, analysis of post-translational modifications, purification of recombinant proteins, protein-protein and protein-DNA interactions, structure prediction, modeling and folding, functional implications of domains and newly emerging investigative methods, allowing analysis of the expression of genes.Selected papers presented at the MPSA 98 are covering new, sensitive and rapid methods for the analysis of proteins, with special emphasis on the total cell proteins, the proteome. In addition to the experimental details, the advantages and limitations of the methodological approaches are discussed.
Topics included are: Protein sequencing analysis, protein and peptide sample preparation, mass spectrometry, NMR, analysis of post-translational modifications, purification of recombinant proteins, protein-protein and protein-DNA interactions, structure prediction, modeling and protein folding, functional implications of protein domains and newly emerging methods for the investigation of the proteome, allowing to analyse the expression of genesI Proteomics and Mass Spectrometry.- 1 Strategies and Methods for Proteome Analysis.- 2 Protein Sequencing or Genome Sequencing. Where Does Mass Spectrometry Fit into the Picture?.- 3 Reversible Negative Staining of Protein on Electrophoresis Gels by Imidazole-Zinc Salts: Micropreparative Applications to Proteome Analysis by Mass Spectrometry.- 4 Direct Analysis of Protein Complexes.- 5 De novo Sequencing of Proteins With Mass Spectrometry Using the Differential Scanning Technique.- 6 Characterization Of Gel Separated Proteins.- II Structure Analysis.- 7 Protein Structure and Dynamics by NMR in Solutló