This volume presents an overview of contemporary quantitative proteomics methods along with instructions on data interpretation, while providing examples on how to implement proteomics into systems biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and practical, Proteomics in Systems Biology: Methods and Protocols is a valuable resource for researchers who are interested in using proteomics techniques to help answer biological and medical questions.
1. Multiplexed Quantitative Proteomics for High-Throughput Comprehensive Proteome Comparisons of Human Cell Lines
Amanda Edwards and Wilhelm Haas
2. Sample Preparation Approaches for iTRAQ Labelling and Quantitative Proteomic Analyses in Systems Biology
Christos Spanos and J. Bernadette Moore
3. Two Birds with One Stone: Parallel Quantification of Proteome and Phospho-Proteome Using iTRAQ
Fiorella A. Solari, Laxmikanth Kollipara, Albert Sickmann, and Ren??P. Zahedi
4. Selected Reaction Monitoring to Measure Proteins of Interest in Complex Samples: A Practical Guide
Yuehan Feng and Paola Picotti
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