Pseudomonas Methods and Protocols [Hardcover]

In Pseudomonas aeruginosa, expert researchers in the field detail many of the methods which are now commonly used to study this fascinating microorganism. Chapters include microbiological methods to high-throughput molecular techniques that have been developed over the last decade. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Pseudomonas aeruginosa aids in the continuing study of new and cutting edge findings.

Part I: BASIC PSEUDOMONAS FEATURES

1. Gene Transfer: Transduction

Emanuela Frangipani

2. Gene Transfer: Transformation/Electroporation

Fr?d?ric Cadoret, Chantal Soscia and? Rom? Voulhoux

3. Gene transfer: Conjugation

Thibault G. Sana, Aur?lie Laubier and Sophie Bleves

4. Pseudomonas Bacteriophage Isolation and Production

Joana Azeredo, Sanna Sillankorva, and ?Diana Priscilla Pires

5. Genotyping Methods

Burkhard Tuemmler

6. Drug Susceptibility Testing by Dilution Methods

Katy Jeannot & Patrick Plesiat

7. Plate-based Assay for Swimming Motility in Pseudomonas aeruginosa

Dae-Gon Ha, Sherry L. Kuchma & George A. OToole

Dae-Gon Ha, Sherry L. Kuchma ?and ?George A. OToole

9. Motility Assay: Twitching Motility

Lynne Turnbull and Cynthia B. Whitchurch

10. Qualitative and Quantitative Asslc)