P-Type ATPases Methods and Protocols [Hardcover]

This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases.  P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, P-Type ATPases: Methods and Protocols aims to ensure successful results in the further study of this vital field of research.

1.      An Introduction to P-type ATPase research

 Poul Nissen

Part I: Protein Production, Isolation, Purification, and Stabilization

2.      Purification of Na,K-ATPase from Pig Kidney

 Natalya U. Fedosova

3.      Preparation of Ca²⁺-ATPase1a Enzyme from Rabbit Sarcoplasmic Reticulum

 Jesper V. M?ller and Claus Olesen

4.      Isolation of H⁺/K⁺-ATPase-enriched Membrane Fraction from Pig Stomachs

 Kazuhiro Abe and Claus Olesen

5.   &nbl“º