Pulse Field Gel Electrophoresis Methods and Protocols [Hardcover]

This? volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

?Part I: Introduction to PFGE

?1. Pulsed-field Gel Electrophoresis for Disease Monitoring and Control

John Besser

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2. Harmonization of PFGE Profile Analysis by using Bioinformatics Tools: Example of the Listeria monocytogenes European Union Reference Laboratory Network

Benjamin F?lix, Sophie Roussel, and Bruno Pot

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3. PFGE as a Tool to Track Listeria monocytogenes in Food Processing Facilities: Case Studies

Marion Dalmasso and Kieran Jordan

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4. Case Study of the use of Pulsed Field Gel Electrophoresis in the Detection of a Food-borne Outbreak

Niall De Lappe and Martin Cormican

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?Part II: PFGE and Pathogenic Bacteria

?5. Pulsed-Field Gel Electrophoresis for Listeria monocytogenes

Laura Luque-Sastre,l³&