Pyrosequencing Protocols [Hardcover]

This book presents detailed protocols for the multidisciplinary application of Pyrosequencing? technology, all written by world-renowned experts. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing?, and supplementing each protocol with troubleshooting tips specific to that method. This volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing?.

Pyrosequencing? Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing? technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing?, and supplementing each protocol with troubleshooting tips specific to that method.

Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing? methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing? technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing? comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing?.

Table of contentsChapter 1: The History of Pyrosequencing?. P?l Nyr?nChapter 2: Pyrosequencing? applications. Sharon MarshChapter 3: Web-Based Primer Design Software for Genome Scale Genotyping by Pyrosequencing?. Steven Ringquist, Christopher Pecoraro, Ying Lu, Alexis Styche,William A. Rudert, Panayiotis V. Benos and Massimo TruccoChapter 4: Pyrosequencing?: A Simple Method for Accurate Genotyping. Cristi R. King and Tiffany Scott-HortonChapter 5: Universal Primer Applications for Pyrosequencing?. Dong-chuan Guo and Dianna M. MilewiczChapter 6: Allele Qual&