Quantum Dots Applications in Biology [Paperback]

Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine as well as enabling applications. Part 3 shows early efforts aimed at using QDs in live animals. The final two parts demonstrate the versatility of QD technology in existing assay technology.

Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine (protein and structural cellular labeling), as well as enabling (single receptor trafficking, clinical pathology, correlative microscopy) applications. Part 3 shows early efforts aimed at using QDs in live animals. The final 2 parts demonstrate the versatility of QD technology in existing assay technology.Table of ContentsPrefaceContributorsPart 1 - Imaging of fixed cells and tissues1. Immunofluorescent Labeling of Proteins in Cultured Cells with Quantum Dot Secondary Antibody ConjugatesRichard L. Ornberg and Hongjian Liu2. Immunohistochemical Detection with Quantum DotsRizwan S. Akhtar, Cecelia B. Latham, Dario Siniscalco, Carlo Fuccio and Kevin A. Roth3. Quantum Dots for Multicolor Clinical Pathology and Multispectral ImagingJohbu Itoh and Robert Yoshiyuki Osamura4. Light and Electron Microscopic Localization of Multiple Proteins using Quantum dotsThomas J. Deerinck, Ben N. G. Giepmans, Benjamin L. Smarr, Maryann E. Martone and Mark H. Ellisman5. Human Metaphase Chromosome FISH using Quantum Dot ConjugatesJoan H.M. KnollPart 2 - Imaging of live cells6. Biotin-Ligand Complexes with Streptavidin Quantum Dots for in vivo Cell Labeling of Membrane ReceptorsDiane S. Lidke, Peter Nagy, Thomas M. Jovin and Donna J. Arndt-Jovin7. Single Quantum Dot Tracking ol�