Receptor and Ion Channel Detection in the Brain Methods and Protocols [Hardcover]

Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, subcellular, anatomical and electrophysiological aspects of the brain. This comprehensive and practical manual is presented in a simple, step-by-step manner for laboratory use, and also offers unambiguous detail and key implementation advice that proves essential for successful results and facilitate choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.

Preface

PART I. MOLECULAR TECHNIQUES
1. Production of high-quality antibodies for the study of receptors and ion channels in brain
Masahiko Watanabe (Hokkaido University School of Medicine, Japan)

2. Co-immunoprecipitation from brain
Xavier Morat?, Dasiel O. Borroto-Escuela, Kjell Fuxe, V?ctor Fern?ndez-Due?as and Francisco Ciruela (Universitat de Barcelona, Spain)

3. Sub-synaptic membrane fractionation
Paula Canas & Rodrigo Cunha (University of Coimbra, Portugal)

4. Investigation of neurotransmitter receptors in brain slices using cell surface biotinylation